Electron microscopy of previously identified cells and processes within the central nervous system.

It is possible to partially transform the silver chromate precipitate present in Golgi stained structures into lead chromate. When this partial replacement is followed by a treatment in a solution containing thiosulphate ions, only lead chromate is left in the tissue. This results in a depleted impregnation of the originally Golgi stained structures without fading of their borderlines. The tissue is then embedded in Araldite. Prior to ultramicrotomy, nerve ceils and other structures can be identified under incident light microscopy. When studied with the electron microscope, they may reveal a relatively preserved cytoplasm within which organelles can be recognized.